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• Electron microscopy • Biochemical assay of brush-border enzymes • Immunocytochemical characterization of B cells, T cells, and their subsets (e.g., CD4 and CD8 cells) and major histocompatibility complex expression by immunohistochemistry and flow cytometry • PCR for cytokine and receptor (nucleotide-binding oligomerization domain, Toll-like receptors) mRNA expression • Assessment of T-cell clonality—PCR for antigen receptor rearrangement (PARR) • Fluorescence in-situ hybridization to demonstrate bacteria in biopsies Empirical Treatment If a specific diagnosis is made, specific treatment(s) can be given
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